Summary of Abstract Submission

Abstract Submission No. IO50-09-0076PresentationPoster

BACTERIAL DIVERSITY FROM OXYGEN DEPLETED ENVIRONMENTS (ODES) OF THE NORTHERN INDIAN OCEAN, UNRAVELLED USING NEXT GENERATION SEQUENCING TECHNIQUE.

Jovitha, V.J.*1, Manohar, C.S.1

1 CSIR National Institute of Oceanography, India

ABSTRACT :

The Arabian Sea and the Bay of Bengal are the two recognised ODEs located in Northern Indian Ocean. Surface sediments were collected from 200m depth off Goa within the ODE of Arabian Sea and from off Paradip within the ODE of Bay of Bengal. Both areas have similar near bottom oxygen profile of ≤ 0.2ml/ L. Total genomic DNA was extracted from sediment sample and were subjected for pyrosequencing. The V1-V4 hyper variable region of 16S rRNA was targeted. A total of 10,069 valid reads corresponding to 955 OTUs from off Goa and 1889 OTUs from off Paradip transect were obtained. The dominant phyla┐s identified include Proteobacteria (33.51%) and Firmicutes (33.21%) from Arabian Sea and Proteobacteria (54.67%) and Planctomycetes (9.48%) from Bay of Bengal. The diversity index like Shannon and Simpson and OTU richness estimator like Ace, Chao 1 and Jacknife indicates the bacterial diversity in ODEs of Bay of Bengal is twice as high as Arabian Sea ODE. The complete sequence identity upto species level was known for 54.8% clones from Arabian Sea and 2.64% clones from Bay of Bengal. Identified isolates have proposed role in biogeochemical cycles, bioremediation, carbon fixation, etc. Bacterial diversity understood from our study reveals that the Bay of Bengal is highly diverse and unexplored in comparison to the Arabian Sea ODE.