Summary of Abstract Submission

Abstract Submission No. IO50-09-0053PresentationPoster

BACTERIAL DIVERSITY AND BIOPROSPECTING IN UNFATHOMED DEEP-SEA SEDIMENTS OF BAY OF BENGAL AND BARREN ISLAND IN ANDAMAN SEA

Pankaj Verma*1, Padmanaban Vishnu Priya2, Rangamaran Vijaya Raghavan1, Gopal Dharani1, Ramalingam Kirubagaran1

1 National Institute of Ocean Technology, India
2 Anna University, India

ABSTRACT :

The marine benthic ecosystems possess bacterial communities of considerable significance adapted to extreme physico-chemical parameters. Here we provide the first picture of bacterial distribution in deep-sea sediments of Bay of Bengal and the volcanic Barren Island in Andaman Sea, using high-throughput Illumina sequencing of 16S rDNA and culture-based diversity. At a genetic distance of 3%, 128715 OTUs were obtained from 8.9 million paired-end raw reads. Actinobacteria was the most dominant phyla representing more than 20% of the OTUs followed by Firmicutes, Proteobacteria and Chloroflexi. Ecological indices and unclassified reads indicate rich bacterial diversity with presence of many new taxa. The rare abundant analysis affirmed that all stations constitute much higher representations of rare phylotypes. The culture-based approach yielded 67 strains grouping into 34 OTUs showing the affiliation to 19 genera of which 40.1% strains are likely to represent new taxa. This is the first report on isolation of Brucella, Fictibacillus, Mesorhizobium and Cobetia spp. from deep-sea sediments. Around 91% of isolates showed at least one or more of the extracellular hydrolytic enzyme activities, such as caseinase, alpha-amylase, urease, gelatinase, lipase and DNase. Among all, Streptomyces was the genera which showed effective antibacterial and anticancer cell line activity. The diversity analysis and the bioactivity assessment highlights that deep-sea sediments are under-explored and could be a hotspot for further investigation and exploitation of the natural compounds derived from deep-sea bacteria.