Summary of Abstract Submission

Abstract Submission No. IO50-09-0048PresentationPoster

NEXT-GENERATION SEQUENCING APPROACH: AN INSIGHT INTO THE SPATIAL VARIATION OF CILIATE DIVERSITY IN THE ARABIAN SEA

Mr.Priya Brata Das*1, Dr.Manguesh Gauns1, Dr.Alexandra Stock2, Dr.Thorsten stoeck2, Dr.SWA Naqvi1

1 National Institute of Oceanography, India
2 Kaiserslautern University, Germany

ABSTRACT :

The next-generation sequencing (NGS) provides an advanced platform to investigate protist (ciliates) diversity in natural environments in a cost effective manner. Studies using 18S rDNA genes from four sites in the Arabian Sea were carried out to identify ciliate community diversity. The results provide the baseline data on ciliate community diversity in response to changes in oxygen levels. The reads of the V4 region derived via NGS, enable an evaluation of ciliate diversity in the open ocean, continental shelf and estuarine waters of the Arabian Sea. V4 tag sequence datasets revealed the taxonomic composition of Colpodea, Litostomatea, Oligohymenophorea, Plagiopylea and Spirotrichea. In the oxic region, Oligotrichia was abundant whereas Cyrtophoria was scarcely recorded. The suboxic zone was dominated by Bryometopida and Stichotrichia were the least abundant.